Plasmid

Part:BBa_I713004:Design

Designed by: Zhenyu Shi   Group: iGEM07_Tsinghua   (2007-10-26)


pEB-AclANcoI-


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2230
    Illegal SpeI site found at 258
    Illegal PstI site found at 1434
    Illegal PstI site found at 2190
    Illegal PstI site found at 3380
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 294
    Illegal SpeI site found at 258
    Illegal PstI site found at 1434
    Illegal PstI site found at 2190
    Illegal PstI site found at 3380
    Illegal NotI site found at 2211
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3167
    Illegal BamHI site found at 252
    Illegal BamHI site found at 1496
    Illegal XhoI site found at 2218
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2230
    Illegal SpeI site found at 258
    Illegal PstI site found at 1434
    Illegal PstI site found at 2190
    Illegal PstI site found at 3380
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2230
    Illegal SpeI site found at 258
    Illegal PstI site found at 1434
    Illegal PstI site found at 2190
    Illegal PstI site found at 3380
    Illegal NgoMIV site found at 1306
    Illegal NgoMIV site found at 2556
    Illegal NgoMIV site found at 3831
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4730
    Illegal BsaI.rc site found at 1835
    Illegal SapI site found at 1053
    Illegal SapI.rc site found at 3680
    Illegal SapI.rc site found at 3890


Design Notes

To ensure the pEASY-B vector can serve as the RAP amplifier vector.


Source

pEASY-Blunt

References