Plasmid
Part:BBa_I713004:Design
Designed by: Zhenyu Shi Group: iGEM07_Tsinghua (2007-10-26)
pEB-AclANcoI-
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 2230
Illegal SpeI site found at 258
Illegal PstI site found at 1434
Illegal PstI site found at 2190
Illegal PstI site found at 3380 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 294
Illegal SpeI site found at 258
Illegal PstI site found at 1434
Illegal PstI site found at 2190
Illegal PstI site found at 3380
Illegal NotI site found at 2211 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3167
Illegal BamHI site found at 252
Illegal BamHI site found at 1496
Illegal XhoI site found at 2218 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 2230
Illegal SpeI site found at 258
Illegal PstI site found at 1434
Illegal PstI site found at 2190
Illegal PstI site found at 3380 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 2230
Illegal SpeI site found at 258
Illegal PstI site found at 1434
Illegal PstI site found at 2190
Illegal PstI site found at 3380
Illegal NgoMIV site found at 1306
Illegal NgoMIV site found at 2556
Illegal NgoMIV site found at 3831 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 4730
Illegal BsaI.rc site found at 1835
Illegal SapI site found at 1053
Illegal SapI.rc site found at 3680
Illegal SapI.rc site found at 3890
Design Notes
To ensure the pEASY-B vector can serve as the RAP amplifier vector.
Source
pEASY-Blunt